User:Andy Maloney/Kinesin & Microtubule Page/PEM-kCT
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Chemicals
PEM
See above page for more information.
PEM-[math]\displaystyle{ \kappa }[/math]C
See above page for more information.
Taxol
See above page for more information.
Recipe
- 50% v/v PEM
- 50% v/v PEM-[math]\displaystyle{ \kappa }[/math]C
- 10 µM Taxol
Notes
This is a dilution of PEM-[math]\displaystyle{ \kappa }[/math]C from 0.5 mg/mL to 0.25 mg/mL with the addition of 10 µM Taxol. This solution is used for the motility assay.
I typically will prepare this every time I go to make a motility solution. Mainly because it requires the use of Taxol and I do not want to have any solution that contains Taxol sitting around for very long.