User:Andy Maloney/Kinesin & Microtubule Page/The alpha4beta1 integrin can mediate leukocyte adhesion to casein and denatured protein substrates
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These are my notes on the paper below. Please read the paper before reading my notes.
- Shows that α4β1 integrin can interact with casein and denatured albumin.
- α4β1 has a binding tripeptide, Leu-Asp-Val (LDV).
- Bovine α-casein does not have a LDV site. But, it does have an aspartic residue that is next to hydrophobic amino acids.
- This is the take home from the paper.
- They note that you need divalent cations for stuff to stick.
- They also note that because you need divalent cations for stuff to stick, this is a big indicator that an integrin is involved in the adhesion.
- I'm not clear on this.
- The possibility of using α4β1 as casein blocker for motility experiments is pretty high. I should look into it.
- They finally note that they used two types of casein fragments, one at 6 kDa and the other at 16 kDa. There are no 16 kDa casein components and so I think that they were looking at some other component in this study.
- The paper was all about α4β1 and how they should look at it more closely to figure out what other kinds of things this integrin will stick to.