User:Andrew W Long/Notebook/CHEM-671 Research/2015/09/08

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UV-Vis and Fluorescence Spectroscopy of Lysozyme Dilutions

Today, each of the 5 groups performed a different dilution of a stock solution of lysozyme and analyzed them with UV-Vis and Fluorescence Spectroscopy. Our group did a 1/10 dilution of the 21.4uM stock (to make a 3mL 2.14 uM solution). The calculations are shown below:

Volume of protein stock taken: 3mL*(1/10) = 0.3mL stock solution Volume of solvent (water): 3*(9/10) = 2.7mL solvent

The resulting graph of the UV-VIS analysis is shown below. UVVis 08 CHEM671 Dilution Practice.png