User:Andrew Perry/Lesser Known Methods in Bacterial Protein Expression
Lesser known methods in recombinant bacterial protein expression
Here are a few lesser known methods / protocols / tricks I've collected over the years. I've love to know if any of them are actually useful for anyone.
Self cleaving TEV protease fusion partner
- <xfeeds pubget="Y">doi:10.1110/ps.041129605</xfeeds>
Thermostable fusion partners
- <xfeeds pubget="Y">doi:10.1016/j.jbiotec.2003.10.008</xfeeds>
Some elastin-like peptides undergo a soluble–insoluble phase transition ("precipitation"), which can be modulated by salt concentration. When used as a fusion partner, it has be shown that this property can be used as a simple purification step.
- <xfeeds pubget="Y">doi:10.1110/ps.04931604</xfeeds>
- <xfeeds pubget="Y">doi:10.1002/pro.157</xfeeds>