Objective
- To prepare packed columns
- To perform column chromatography on BPA solutions from [User:Michael_S._Bible/Notebook/581/2014/10/22| Oct. 22]
Preparation of packed column
- Place glass wool on bottom of column (we are using 50 mL syringes as columns)
- Cover with a layer of sand
- Add silica packing material prepared by dissolving 10 g of silica in methanol
- Allow to settle
- Rinse with water to remove methanol
Analysis of 10.4 ppm BPA stock solution
- Added 10 mL of BPA solution to the column
- Added 10 mL water to the column
- Added 30 mL of methanol to the column
- Collected eluted liquid at 5 mL intervals
- Spotted on TLC plate, and using UV light, checked for fluorescence.
Results
None of the spots glowed when dried and placed under a UV light. It is likely that the solution was not concentrated enough to show up under UV light. A new method for determining the concentration of BPA must, therefore, be devised.
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