User:Andrew K Farag/Notebook/CHEM-581/2014/10/24

Biomaterials Design Lab

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Objective To prepare packed columns To perform column chromatography on BPA solutions from [User:Michael_S._Bible/Notebook/581/2014/10/22| Oct. 22] Preparation of packed column Place glass wool on bottom of column (we are using 50 mL syringes as columns) Cover with a layer of sand Add silica packing material prepared by dissolving 10 g of silica in methanol Allow to settle Rinse with water to remove methanol Analysis of 10.4 ppm BPA stock solution Added 10 mL of BPA solution to the column Added 10 mL water to the column Added 30 mL of methanol to the column Collected eluted liquid at 5 mL intervals Spotted on TLC plate, and using UV light, checked for fluorescence. Results None of the spots glowed when dried and placed under a UV light. It is likely that the solution was not concentrated enough to show up under UV light. A new method for determining the concentration of BPA must, therefore, be devised.