User:Andrew K Farag/Notebook/CHEM-571/2014/02/25

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Protocol

  • Run fluorescence spectroscopy for the previously synthesized sets: Cys, GSH, 1-Thioglycerol.
  • Finish precipitation of 80% BSA-CYS sample

Description

The solutions created for tomorrow:

  1. 250mL of 20mM L-cysteine
    1. FW 121.16g/mol
    2. measure out: 0.6058g
    3. measured: 0.6063g
    4. actual conc: 19.98mM
  2. 50mL of 100mM ZnSO4
    1. FW 287.56g/mol
    2. measure out: 1.4378g
    3. measured: 1.4399 g
    4. actual conc: 100.1 mM
  3. 50mL of 10mM MnCl2
    1. FW 197.9g/mol
    2. measure out: 0.09895g
    3. measured: 0.0987g
    4. actual conc: 9.98 mM
  4. 10mL 1M NaOH
    1. FW 40.00g/mol
    2. 0.4284g
    3. Final concentration a bit over 1M check pH carefully when adjusting!
  5. 50mL 100mM Na2S
    1. FW 240.18g/mol
    2. measure out: 1.2009g
    3. measured: 1.2007g
    4. actual conc: 100 mM

Data

  • Add data and results here...

Notes


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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