Objective
- Synthesize Pepsin linked AuNP's as shown by Dr.Hartings HERE.
- Calculate the concentration ratio for Pepsin/AuNP and HRP-AuNP fiber formation.
Procedure
Ratios were made as follows
- These ratios allow a constant concentration of Gold at .25mM, and corresponding protein concentration.
- The amounts were measured using micropipettes.
- Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.
- Once prepared, the test tubes were covered with foil and were baked for 4hrs at 80 degrees Celsius.
Data
- No data was taken today!!
|