UCLAiGEM:Protocols/degumming

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<html> <h1>Silk Degumming</h1>

<h3>Reference: <a href = "http://www.nature.com/nprot/journal/v6/n10/full/nprot.2011.379.html">"Materials fabrication from Bombyx mori silk fibroin," Kaplan 2011 </a></h3>


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Longer boiling time will degrade the fibroin.</span></li></ol><ol class="c2 lst-kix_ou0i81wz8g97-0" start="5"><li class="c1"><span>Remove the silk using a spatula and rinse it in cold ultrapure water</span></li><li class="c1"><span>Squeeze excess water from the silk</span></li><li class="c1"><span>Place silk in 1L of ultrapure water</span></li><li class="c1"><span>Stir with a stir bar for 20 minutes</span></li><li class="c1"><span>Repeat steps 6-8 twice for a total of 1 hr of rinsing</span></li><li class="c1"><span>Remove silk, squeeze out water, and spread out on a clean sheet of aluminum foil</span></li><li class="c1"><span>Allow to dry overnight in a fume hood</span></li></ol></body></html>



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