Turner:Solutions/Stocks

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Stocks Conc. Units FW (g/mol) Vol stock (L) g to add Use Notes Last made
NaCl 5 M 58.44 0.50 146.100 Stock autoclave 10/1/12
Tris-HCl pH 8 1 M 121.140 0.50 60.570 Tris Base pH with ~17 mL of 12.1N HCl, autoclave 10/1/12
EDTA pH 8 0.5 M 372.240 0.50 93.060 Na Salt pH with NaOH, autoclave 10/1/12
SDS 20 % N/A 0.10 20.000 Stock do not sterilize 10/1/12
Tris-HCl pH 7.4 1 M 121.140 0.50 60.570 Tris Base pH with ~20 mL of 12.1N HCl, autoclave 10/1/12
DTT 0.1 M 154.250 0.02 0.309 f.s., 1 mL aliquots, store at -20C 10/1/12
Maltose 0.5 M 342.300 0.10 17.115 Stock f.s. 10/1/12
HEPES pH 7.9 1 M 238.300 0.10 23.830 Stock pH with ~3 mL 10N NaOH, f.s. 10/1/12
MgCl2 1 M 203.300 0.100 20.330 Hexahydrate autoclave 10/1/12
MnCl2 1 M 197.910 0.100 19.791 Tetrahydrate autoclave 10/1/12
Glycerol 50 % N/A N/A N/A Lab stock N/A