Turner:Solutions/GAMBITTWB

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GAMBIT TWB - 50 mL

Component Final [] Stock [] Units mL stock ' '
Tris-HCl pH 7.4 0.02 1 M 1
NaCl 0.2 5 M 2
EDTA pH 8 0.001 0.5 M 0.1
DTT 0.002 0.1 M 1
Glycerol 10 50 % 10
pH to 7.4 with 5N HCl (approx) 2 drops
sterilize by f.s.
store at 4C
Note: add 1 tablet cOmplete mini, EDTA-free to 10 mL buffer before use