Turner:Solutions/GAMBITMgRxn

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GAMBIT 2x Reaction Buffer + Mg - 10 mL

Component Final [] Stock [] Units mL stock ' '
Glycerol 20 50 % 4
HEPES pH 7.9 0.05 1 M 0.5
DTT 0.004 0.1 M 0.4
NaCl 0.2 5 M 0.4
MgCl2 0.01 1 M 0.1
pH to 7.9 with _ (approx) _ mL
sterilize by f.s.
store at 4C
Note: use 100x BSA from NEB as 40x stock for GAMBIT reaction