Tk:video protocols

• Making plates / Media
  • antibiotics
  • weighing
  • autoclaving
  • pH
  • water bath / prevention of stuff growing
  • sterile technique
  • material
  • sterile filtration
  • defined media

• Growing cultures
  • spreaders / glass beads
  • biosafety
  • rotators
  • aeration
  • pH
  • indicators
  • temperature
  • OD measurements
  • phases of growth

• Receiving and sending strains and plasmids
  • recovering plasmids from spotted or dried DNA
  • growing stab cultures
  • preserving cultures in glycerol
  • preserving cultures with stabs
  • preserving cultures on plates
  • sending DNA
  • sending stabs
  • sending dry ice samples

• Labels and lab notebooks
  • dated / initialed labels
  • lab notebooks

• Agarose gels
  • percentage choice
  • buffer choice
  • microwaving the gel
  • adding dye
  • pouring the gels
  • bottle cleanup
  • running the gel
  • buffer choice
  • adding dye to the buffer
  • running time
  • other kinds of agarose
  • loading dye
  • dna markers
  • visualizing the bands
  • UV exposure issues

• Cutting DNA from agarose gels
  • UV exposure issues
  • weighing gel samples
  • isolation of DNA from gel slices

• PCR reactions
  • primer design
  • reaction components
  • setting up the reaction
  • choice of cycling conditions
  • hot lids