Timp:stickydishprotocol

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Sticky Film (TMPM) Dish Treatment Protocol

  • Materials:
    • 200 Proof Ethanol
    • H2O (MilliQ)
    • Glacial Acetic Acid
    • 3-(trimethoxysilyl) propyl methacrylate, 98%
  • Procedure (To treat approximately 9 dishes):
  1. Add 9.5 mL 200 Proof Ethanol to vial
  2. Add 500 µL H2O (MilliQ)
  3. Remove 50 µL solution - add 50 µL Glacial Acetic Acid
  4. Remove 200 µL solution - add 200 µL 3-(trimethoxysilyl) propyl methacrylate
  5. Add 1 mL to each dish - filter sterilized through a .22 µm syringe filter
  6. Wash twice with MilliQ water.
  7. Sterilize with 70% Ethanol, aspirate and let dry/sterilize under UV in TC hood