Timp:hydrogelProtocol

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Hydrogel Protocol

Protocol for preparing PEGDA hydrogel, polymerizes under UV light.

Materials

  • PEGDA (Poly ethylene glycol diacrylate)
    • Acrylate-PEG-Acrylate-3400 (Laysan Bio, Inc.)or ACRL-3400 (NEKTAR 01010F12)
  • Argon gas
  • M9 Media (no antibiotics)
  • Photo Initiator (2-Hydroxy-2-methyl-propiophenone, 97%, Sigma Aldrich 405655)
  • 1.5ml Microcentrifuge Tubes (Fisher Scientific)

Making PEGDA

  1. Weigh out 100 mg PEGDA into microcentrifuge tubes.
    • Note: If storing, close container inside glove bag while flowing argon and store in freezer.
  2. Add 10% wt/vol of M9 media
  3. Spin at 10krpm for 20 minutes.
  4. Store in Fridge

Sample Preparation

  1. Add 100 μL of PEGDA solution to micro-centrifuge tube
  2. Add 0.3 μL of photo initiator and vortex mix for 1 minute
  3. Add sample and pipette up and down gently.