Timp:hydrogelProtocol

Home        Contact        Protocols        Lab Members        Publications        Research       

Hydrogel Protocol

Protocol for preparing PEGDA hydrogel, polymerizes under UV light.

Materials

• PEGDA (Poly ethylene glycol diacrylate)
  • Acrylate-PEG-Acrylate-3400 (Laysan Bio, Inc.)or ACRL-3400 (NEKTAR 01010F12)
• Argon gas
• M9 Media (no antibiotics)
• Photo Initiator (2-Hydroxy-2-methyl-propiophenone, 97%, Sigma Aldrich 405655)
• 1.5ml Microcentrifuge Tubes (Fisher Scientific)

Making PEGDA

1. Weigh out 100 mg PEGDA into microcentrifuge tubes.
   • Note: If storing, close container inside glove bag while flowing argon and store in freezer.
2. Add 10% wt/vol of M9 media
3. Spin at 10krpm for 20 minutes.
4. Store in Fridge

Sample Preparation

1. Add 100 μL of PEGDA solution to micro-centrifuge tube
2. Add 0.3 μL of photo initiator and vortex mix for 1 minute
3. Add sample and pipette up and down gently.