Integrases/Excisionases pairs to test

From OpenWetWare
Jump to: navigation, search

We will start to test 3 pairs of the serine integrase family. Integrases from this family are interesting because they do not require specific cofactors and have been shown to work in a wide range of species. These 3 pairs are the best characterized members from the family which have an identified excisionase.

Bxb1

From Mycobacterium phage Bxb1.

Uniprot entry:

Bxb1 integrase Bxb1 excisionase

References:

[1], [2], [3].

From Lactococcus lactis phage TP901-1.

Uniprot entries:

TP901-1 integrase TP901-1 excisionase

Structure of the catalytic domain: [3BVP]

References: [4], [5], [6], [7].


From Mycobacterium tuberculosis prophage-like element phiRv1.

Uniprot entries:

phiRV1 integrase phiRV1 excisionase

References: [8], [9]

References

[1] Genome organization and characterization of mycobacteriophage Bxb1., mediavilla et al., Mol microbiol, 2000. PMID 1123671.

[2] Control of phage Bxb1 excision by a novel recombination directionality factor., Ghosh P. et al, PLoS Biol., 2006. PMID: 16719562.

[3] Phage Bxb1 integrase mediates highly efficient site-specific recombination in mammalian cells., Russel JP et al., Biotechniques, 2006. PMID:16629393

[gamma delta resolvase Uniprot]