Template:SBB10 ConstructionFiles ZhenH sbb31
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sbb31: CA42 origin of replication
Source: pEC22-CA42
Target Sequence: agcacttcagcgcgccgtagcatcgataaacattacgggatggggcgaaactgccatctgttcgaaatgacgcgcaaatgggcttacagggcgattcgtcagggctggccagcattctcacagtggcttgatgccgtgattcagcgtgtcgaaatgtacaacgcatcgcttcccgttccgctttcacctcctgaatgtcgggctattggcaagagtattgcgaaatacacgcacaggaacttcacggcggaaactttcgcacagtatgtggctgatacgcacacgccagaaatacaggccaagagaggcaggaaaggtggcatcgctaaaggcgaagcctacgatgacaagcgtttcatggcgctatgtatgctggagaatggatattctcagaaagctattgcggcgatgttggaggtttctactcgaaccattcgaaactggaaaagcggaaaatagcctatatcagataacagcgcctttctggcgtttttttgagcagtaggtcttttgccg
Vector: pBjk2741
Short description: oriCA42
Genbank reference: D30056.1
Family: Origin of Replication
PCR ZZH001 and ZZH002 on pEC22-CA42 (547 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1144 (EcoRI/BamHI, 2472+910, L)
Product is pBca9523-sbb31 {oriCA42}
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ZZH001 Forward oligo for cloning of oriCA42
ccataGAATTCatgAGATCTagcacttcagcgcgccgtag
ZZh002 Reverse oligo for cloning of oriCA42
ctgatGGATCCcggcaaaagacctactgctc
JCA Notes
- Correct, except should clone into pBjk2741-Bca1144
PCR ZZH001 and ZZH002 on pEC22-CA42 (547 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 2472+910, L) Product is pBjk2741-sbb31 {oriCA42} ---------------------------- ZZH001 Forward oligo for cloning of oriCA42 ccataGAATTCatgAGATCTagcacttcagcgcgccgtag ZZh002 Reverse oligo for cloning of oriCA42 ctgatGGATCCcggcaaaagacctactgctc
