Template:SBB10 ConstructionFiles RaffiH sbb09

Construction of Tn5 3'TR (sbb09) BglBrick Part

  EIPCR rh01F/rh02R on pBjk2741-Bca1144   (2230 bp, BglII) 
  Product is pBjk2741-sbb09               {Tn5 3'TR}
  ----
  rh01F  EIPCR construction of Tn5 3'TR basic part  ccataAGATCTggttgagatgtgtataagagacagtcgacGGATCCtaactcgctcctcag
  rh02R  Reverse BglII oligo for pBjk2741 EIPCR     ccaatAGATCTcatGAATTCcacttcag

JCA Notes

• correct
• similarity to other parts, changed construction:

Construction of Tn5 3'TR (sbb09) BglBrick Part

  EIPCR rh01F/tn0002R on pBjk2741-Bca1144   (2230 bp, BglII) 
  Product is pBjk2741-sbb09               {Tn5 3'TR}
  ----
  rh01F  EIPCR construction of Tn5 3'TR basic part
ccataAGATCTggttgagatgtgtataagagacagtcgacGGATCCtaactcgctcctcag
 tn0002R	Reverse BglII oligo for Tn5 EIPCR 
 CCAATAGATCTcatgaattcCACTTCAG