Template:SBB10 ConstructionFiles MaziarB sbb19
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Construction of FokI Cleavage Domain basic part sbb19 PCR mb0001/mb0003 on BBa_K157000 (504 bp, gp = A) PCR mb0002/mb0004 on BBa_K157000 (143 bp, gp = B) --------------------------------------------------- PCR mb0001/mb0004 on A+B (622 bp, EcoRI/BamHI) Digest pBcajk2741-Bca1144 (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb19 {<FokI+!} --------------------------------------------------- mb0001 Forward EcoRI + BglII ctacgGAATTCgctAGATCTCAGCTGGTTaaatctgaactggaggag mb0002 Forward point mutation A-->T cataaaaccaatTgcaatggcgccg mb0003 Reverse point mutation A-->T cggcgccattgcAattggttttatg mb0004 Reverse BamHI gtacgGGATCCTTAaaaattgatctccccattg
JCA Notes
- Fixed wiki link
- Prefix should be ATG not GCT
- Changed template name to partName
- Construction file is correct otherwise
- Changing some oligos for sharing with another part
Construction of FokI Cleavage Domain basic part sbb19 PCR fk0001/fk0003 on BBa_K243000 (504 bp, gp = A) PCR fk0004/mb0002 on BBa_K243000 (144 bp, gp = B) --------------------------------------------------- PCR fk0001/mb0002 on A+B (622 bp, EcoRI/BamHI) Digest pBjk2741-Bca1144 (EcoRI/BamHI, 2170+910, L) Product is pBjk2741-sbb19 {<FokI+!} --------------------------------------------------- fk0001 Forward EcoRI and BglII for FokI ccaaaGAATTCatgAGATCTCAGCTGGTTaaatctgaactggaggag fk0004 (F)Base Substitution FokI ccataaaaccaatTgcaatggcgccg fk0003 (R)Base Substitution FokI cggcgccattgcAattggttttatgg mb0002 Reverse BamHI gtacgGGATCCTTAaaaattgatctccccattg