Template:SBB10 ConstructionFiles KaiM sbb16

 sbb16: phiC31 attP

 Wobble KRM003/KRM004            (115bp, EcoRI/BamHI)
 Sub into pBjk2741-Bca1144         (EcoRI/BamHI, 2170+910, L)
 Product is pBjk2741-sbb16     {phiC31 attP}
 ----
 KRM003   Forward construction of phiC31 attP basic part
 ccataGAATTCatgAGATCTagaagcggttttcgggagtagtgccccaactggggtaacctttgag
 KRM004   Reverse construction of phiC31 attP basic part
 ctgatGGATCCgtgtcatgtcggcgaccctacgcccccaactgagagaactcaaaggttaccccagttg

JCA Notes

• correct
• Entry modified on clotho, seq included eco, bam, and tails