Template:SBB10 ConstructionFiles JoseG sbb38

Biobricking of Pbad

PCR JG003-F and JG004-R on pBca1100-Bca1152.    (276 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is Pbad-pBjk2741    {Pbad-J23100}
----------------------------
JG003-F   Cloning of papC   ccataGAATTCgtccAGATCTaaccaattgtccatattgcatcag
JG004-R   Cloning of papC   ctgatGGATCCgctaatcttatggataaaaatgc

JCA Notes

• This was a little misleading, we actually want AraC-Pbad, not just the pbad promoter itself. This construction file would adequately make a Pbad-only part
• Nomenclature of plasmid changed
• Modified entry on clotho

Eco/Bam transfer pBjh1601CK-ig114
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-ig114   {AraC-Pbad}