Template:SBB10 ConstructionFiles JoseG sbb37

Biobricking of Pcon

PCR JG005-F and JG006-R on pBca1100-Bca1152    (66 bp, EcoRI/BamHI)
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is Pcon-pBjk2741    {Pcon-J23100}
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JG005-F   Cloning of papC   ccataGAATTCgtccAGATCTttgacggctagctcagtcct
JG006-R   Cloning of papC   ctgatGGATCgctagcactgtacctaggac

JCA Notes

• This just needs to be eco/bam transferred, not pcr'd
• Nomenclature of plasmid changed
• Modified entry on clotho

Eco/Bam transfer pBca1100-Bca1152
Sub into pBjk2741-Bca1144                      (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bca1152   {Pcon-J23100}