Template:SBB10 ConstructionFiles JoseG sbb30
From OpenWetWare
Jump to navigationJump to search
Biobricking of O99 rep Gene
PCR Pcon-F and Pcom-R on pEC52. (1236 bp, EcoRI/BamHI) Sub into pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is Pcon-pBjk2741 {5'UTR_repO99!} ---------------------------- JG001-F Cloning of O99 rep Gene ccataGAATTCgtccAGATCTaaaaacgattctgacgcatttt JG002-R Cloning of O99 rep Gene ctgatGGATCCctactctacaagacctcgtttt
JCA Notes
- Needs to be with vector pBca9523
- Oligo names are incorrect in description
- Extended oligos to get to a g/c
- Name of plasmid should be vectorName-partName (pBca9523-sbb30)
- Corrected Clotho entry
- Otherwise correct
Biobricking of O99 rep Gene PCR JG001-F and JG002-R on pEC52 (1235 bp, EcoRI/BamHI) Sub into pBca9523-Bca1144 (EcoRI/BamHI, 910+2472, L) Product is pBca9523-sbb30 {5'UTR_repO99!} ---------------------------- JG001-F Cloning of O99 rep Gene ccataGAATTCatgAGATCTaaaaacgattctgacgcattttttatg JG002-R Cloning of O99 rep Gene CTGATGGATCCCTACTCTACAAGACCTCGTTTTttc