Template:SBB10 ConstructionFiles JeniL sbb34
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Construction of Ala6 basic part sbb34 EIPCR Ala6-F/ca2741R on pBjk2741-Bca1144 (2203 bp, BglII) Product is pBjk2741-Ala6 {<Ala6>} ---- Ala6-F EIPCR construction of Alanine-6 part CCATAagatctgcagcagcagcagcagcaGGATCCtaaCTCGCTCCTCagg jl2741R Reverse BglII oligo for Ala6 EIPCR on pBjk2741-Bca1144 CTGAAGTGgaattcatgAGATCTATTGG
JCA Notes
- Reverse oligo is reverse complemented
- Should name plasmid as vectorName-partName, not vectorName-shortDescription
- Due to similarity to other parts, reusing an oligo
- I've also mutated out the psti for compatibility with other standards
Construction of Ala6 basic part sbb34 EIPCR Ala6-F/tn0002R on pBjk2741-Bca1144 (2219 bp, BglII) Product is pBjk2741-sbb34 {<Ala6>} ---- Ala6-F EIPCR construction of Alanine-6 part CCATAagatctgcTgcCgcagcagcagcaGGATCCtaaCTCGCTCCTCagg tn0002R Reverse BglII oligo for Tn5 EIPCR CCAATAGATCTcatgaattcCACTTCAG Part: gatctgcTgcCgcagcagcagcaG