Template:SBB10 ConstructionFiles DorothyT sbb25

Construction File of sbb25
PCR sbb25-F and sbb25-R on pEC22-CA42    (661 bp, EcoRI/BamHI)
Sub into pBca9523-Bca1455              (EcoRI/BamHI, 2472+915, L)
Product is pBca9523-sbb25
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sbb25-F   Cloning of sbb25   ccataGAATTCatgAGATCTcagctgaagtgaccggattagc
sbb25-R   Cloning of sbb25   ctgatGGATCCgcacgccctttgccggagatgcgaag

JCA Notes

• Correct