Template:SBB10 ConstructionFiles DanielS sbb40

EIPCR DS005/DS006 on pBjk2741   (2212 bp, EcoRI)
Product is pBjk2741-NIS    {<NIS!}
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DS005      EIPCR construction of NIS Bglbrick basic part
gaatccCTCagatctCCGCCGAAAAAAAAACGTAAAGTTtaaggatcctaaCTCGAGctgcag
DS006    Reverse oligo for NIS EIPCR
CCAATAGATCTcatgaattccagaaatc

JCA Notes

• Correct, but you can do this one by eco/bam transfer
• Need to put part into Clotho -- call it Bjh1858, not sbb40

Eco/Bam transfer pBjh1601CK-Bjh1858
Sub into pBjk2741-Bca1144            (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-Bjh1858  {<NIS!}