Template:SBB10 ConstructionFiles Chel sbb06
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sbb06: piggieBac 3'TR
Pool MNF001 through MNF008, assemble by PCA
PCR MNF01/MNF10 on PCA reaction (267 bp, EcoRI/BamHI)
Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L)
Product is pBjk2741-sbb06 {<piggieBac-3'TR>}
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MNF01 PCA Assembly of sbb06 CCAGTGAATTCGTCCAGATCTTTTGTTACTTTATAGAAGAAATT
MNF02 PCA Assembly of sbb06 TTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAA
MNF03 PCA Assembly of sbb06 TTTTGTTTTTTTTTAATAAATAAATAAACATAAATAAATTGTTTG
MNF04 PCA Assembly of sbb06 CACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTT
MNF05 PCA Assembly of sbb06 TATTATTAGTATGTAAGTGTAAATATAATAAAACTTAATATCTAT
MNF06 PCA Assembly of sbb06 ATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATT
MNF07 PCA Assembly of sbb06 AATAAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAA
MNF08 PCA Assembly of sbb06 TACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATC
MNF09 PCA Assembly of sbb06 CATGATTATCTTTAACGTACGTCACAATATGATTATCTTTCTAGG
MNF10 PCA Assembly of sbb06 GCAGTGGATCCTTAACCCTAGAAAGATAATCATAT
JCA Notes
- Correct except for prefix sequence should be atg
sbb06: piggieBac 3'TR Pool MNF001 through MNF008, assemble by PCA PCR MNF01/MNF10 on PCA reaction (267 bp, EcoRI/BamHI) Sub in pBjk2741-Bca1144 (EcoRI/BamHI, 910+2170, L) Product is pBjk2741-sbb06 {<piggieBac-3'TR>} ------------- MNF01 PCA Assembly of sbb06 CCAGTGAATTCatgAGATCTTTTGTTACTTTATAGAAGAAATT MNF02 PCA Assembly of sbb06 TTATTAAAAAAAAACAAAAACTCAAAATTTCTTCTATAAAGTAA MNF03 PCA Assembly of sbb06 TTTTGTTTTTTTTTAATAAATAAATAAACATAAATAAATTGTTTG MNF04 PCA Assembly of sbb06 CACTTACATACTAATAATAAATTCAACAAACAATTTATTTATGTT MNF05 PCA Assembly of sbb06 TATTATTAGTATGTAAGTGTAAATATAATAAAACTTAATATCTAT MNF06 PCA Assembly of sbb06 ATATCGAGGTTTATTTATTAATTTGAATAGATATTAAGTTTTATT MNF07 PCA Assembly of sbb06 AATAAATAAACCTCGATATACAGACCGATAAAACACATGCGTCAA MNF08 PCA Assembly of sbb06 TACGTTAAAGATAATCATGCGTAAAATTGACGCATGTGTTTTATC MNF09 PCA Assembly of sbb06 CATGATTATCTTTAACGTACGTCACAATATGATTATCTTTCTAGG MNF10 PCA Assembly of sbb06 GCAGTGGATCCTTAACCCTAGAAAGATAATCATAT
