Template:SBB10 ABMarker

From OpenWetWare
Jump to: navigation, search

This part encodes an antibiotic marker

Antibiotic resistance proteins confer resistance to some specific, toxic small molecule. This part is a composite part containing a complete promoter.rbs.cds.term composite part. So, when you introduce it into the cell it should confer resistance to the cognate antibiotic.

Complete genes are often toxic to E. coli, so you may have some difficulty making this part. Make careful note in your notebook about the size distribution of colonies you get on your plates and the ratio of red to white colonies. When building your part, you should plate twice. On one plate, use just the antibiotic associated with your vector (usually spectinomycin). On a second plate, use both the vector antibiotic and the antibiotic associated with this part. Note how many colonies are observed on the plates in your notebook.