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Welcome to your project page!
For each part listed, you should:
- Design oligos to make your part
- Write up proper construction files and put it on the Construction Files page
- Put your oligos on the Oligo Log page
- Put your part sequence on the part document
- Name your parts according to the list here
upaG Autotransporter short version
Source: CFT073 genomic DNA
First of all, read PMID: 18424525. Also download this template for your design here. You are going to make the part that includes only the "L1 L2 beta" region (red).
This part encodes a N-terminal display protein
Your displayer part should be of the {<part!} style (no start, with stop). There should be NO prepro sequence in your part. You should design your construction file to insert your part into plasmid pBca9495KC-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.
Hydroxyapatite-binding peptide
Source: synthetic
You should read PMID: 18701808 for background. You are going to make a part encoding the peptide:
APWHLSSQYSRT
No start, no stop, in frame.
This part encodes a passenger protein
Your passenger part should be of the {<part>} style (no start, no stop). There should be NO prepro sequence in your part. If your part is naturally secreted, you should encode only the active peptide. You should design your construction file to insert your part into plasmid pBca9495AK-Bca1144#5 using EcoRI and BamHI. The map of this plasmid is here.
