Template:PCR reaction

Reaction 1

Reaction Mix (μl)		Thermocycling
H2O	{{{Water}}}	{{{Denaturing Temp}}}	{{{Denaturing Time}}}
Buffer	{{{Buffer}}}	{{{Denaturing Cycle Temp}}}	{{{Denaturing Cycle Time}}}
Forward Primer	{{{PrimerF}}}	{{{Annealing Temp}}}	{{{Annealing Time}}}
Reverse Primer	{{{PrimerR}}}	{{{Extension Temp}}}	{{{Extension Time}}}
dNTPS	{{{dNTPs}}}	Cycles	{{{Cycles}}}
Template	{{{Template}}}	{{{Final Extension Temp}}}	{{{Final Extension Time}}}
Polymerase	{{{Polymerase}}}	4oC	{{{4CTime}}}
{{{Notes}}}