# Template:AndersonLab:BBb Standard

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### The BglBrick Format

The BglBrick standard was developed by several researchers at UC Berkeley and is based on idempotent assembly with BamHI and BglII restriction enzymes. In a nutshell, most plasmids look like this:

 GAATTCatgAGATCT-part-GGATCCtaaCTCGAG


and BglBrick scars are "GGATCT" encoding gly-ser when translated in frame. Note, however, that BglBrick is intended as a minimal physical assembly standard, and only those features needed for interconversion of BglBrick plasmids are formally defined. Therefore, "atg" and "taa" spacers are not core definitions of the standard.

The current method for expressing BglBrick parts in the Registry is to make them all basic parts with the sequence in between BglII and BamHI. So, all parts, basic, composite, and intermediate, are expressed as Basic Parts.

Formal Definition:

• A BglBrick part is a DNA sequence flanked on the 5' end by "GATCT" and on the 3' end by "G" lacking BglII, BamHI, EcoRI, and XhoI restriction sites
• A BglBrick vector is a DNA sequence flanked on its 5' end by "GATCC" and on its 3' end by "A"
• A BglBrick entry vector has a unique EcoRI site, no BamHI or BglII restriction sites, and at most one XhoI site 5' to the EcoRI site
• A BglBrick plasmid is represented as <vector_name>-<part_name> and has the sequence obtained by concatenating the vector and part sequences
• Further definition constraints are "sub-standards" of the BglBrick format