Talk:Transforming chemically competent cells
Amount of plasmid DNA used
I have a few suggestions:
- the amount of plasmid used should better be posted in µg. Are there any recommendations for the exact amount?
- There are a lot of abbreviations (TSS) used. maybe this should be clarified as well.
--Mathias Klode 08:24, 16 April 2009 (EDT)
In my hands, shaking can reduce the transformation efficiency, specially for Ampicillin resistance.
- zurdo 07:06, 28 April 2009 (EDT):
Shaking at which stage exactly? And I don't think there is an interdependency between transformation efficiency, antibiotic and shaking... (damn I forgot how to change indentation) --Mathias Klode 07:23, 28 April 2009 (EDT)