Talk:Prbbbb:small scale expression v1

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I have tried this protocol-I am seeing no difference between uninduced and induced cells after adding glucose so I wonder if induction could be done in 2YT without glucose.I also wanted to know why 10 micromolar IPTG is recommended.I saw another reference in Protein Peptide and Science 2006 about use of 1 to 10 micromolar IPTG?What is the logic of this? Awaiting replies eagerly Gautam Krishnan