Talk:BE.109:Systems engineering/Basic bacterial photography: black and white

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β galactosidase assay results

You should post your results and calculations from your β-galactosidase assays here.

Tuesday/Thursday section

Team color Sample name OD600nm Average A420 Average A550 Calculated units
Green Light 1:10 .1397 .1231 .029 60.05
Green Dark 1:10 .1743 .2477 .0315 149.11
Blue! light 1.5908 0.2578 0.0424 985
Blue! dark 1.9095 0.8475 0.1426 3809
Purple light, undil. 2.224 0.761 0.280 315
Purple light, 1:10 0.2224 0.173 0.0053 516
Purple dark, undil. 3.218 1.56 0.372 806
Purple dark, 1:10 0.3218 0.282 0.071 1337
Yellow light 1.7875 0.38 0.06 862.85
Yellow dark 1.6502 0.83 0.27 929.79
Red light 1:100 1.81 0.108 0.0242 505.33
Red light 1:10 3.008 0.831 0.0585 337.5
Red dark 1:100 1.77 0.200 0.0232 1255.21
Red dark 1:10 2.855 0.248 0.0781 189.91

Wednesday/Friday section

Team color Sample name OD600nm Average A420 Average A550 Calculated units
Green light 1:5 .3245 .0217 122
Green light 1:10 .2607 .0056 122
Green dark 1:5 .404 .0145 196
Green dark 1:10 .3717 .0061 206
Purple light 1:2 1.0397 .4403 .0122 1885000
Purple light 1:5 .5247 .2612 .0229 4076000
Purple dark 1:2 .784 .3285 .0814 4923000
Purple dark 1:5 .4997 .3763 .041 7300000
Yellow Light (1:10) 0.3220 0.8358 0.0054 987
Yellow Dark (1:10) 0.3426 0.4435 0.0221 400
Pink light 1:10 0.3255 0.2439 0 431.8
Pink light 1:20 0.1672 0.2424 0 420.1
Pink dark 1:10 0.2839 0.3958 0 1161.8
Pink dark 1:20 0.1472 0.3125 0 1158.0
Blue Light 1:5 0.6878 0.3559 0.0066 361.93
Blue Light 1:10 0.3681 0.2172 0.0034 362.30
Blue Dark 1:5 0.5652 0.5625 0.0213 1052.2
Blue Dark 1:10 0.3111 0.3747 0.0056 991.06
Red Light 1:2 1.2552 0.467 0.024 427.887
Red Light 1:5 0.5504 0.302 0 539.875
Red Dark 1:2 0.8589 0.479 0 608.855
Red Dark 1:5 0.3282 0.391 0.012 1734.090


Tips for next year

  1. Think about the order in which you do your assemblies. Short pieces of DNA tend to be hard to manipulate experimentally. Plan your assembly such that you cut out the large fragments from plasmids and leave the small fragments as part of the plasmid.
  2. Using more cells in your bacterial photograph seems to lead to poorer contrast but better resolution.
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