Talk:BE.109:Protein engineering/Purifying beta-galactosidase

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To compare the purification protocols you have tried today, each team in each lab section should enter their data into the following tables.


Tuesday/Thursday

Team Color purification method equation for BSA standard curve concentration of beta-gal stock concentration of purified beta-gal (wild type) activity of purified beta-gal (wild type) concentration of modified beta-gal (list modification below table) activity of modified beta-gal
Green Ni-agarose y=.7133x - .0244 2.08 mg/ml .29 mg/ml 27595 units .158 mg/ml 1822 units
Purple Paramagnetic Beads OD-595 = 0.5556(Conc.) - 0.0012 4.19 mg/mL 0.0501 mg/mL 37.37 units 0.0506 mg/mL 34.87 units
Red Ni-agarose y=.7893x-0.0274 2.345 mg/mL 0.874 mg/mL .076 .56 mg/mL .085
Blue! Ni-agarose 0.6709x-0.0002 3.75mg/mL 0.647 0.0318 0.322 0.233
Pink Ni-agarose y=1.2x+0.0002 2.332 mg/mL 0.111 mg/mL OD 420= 0.7363 , Units = 2.6783 0.268 mg/mL OD 420= 0.2173, Units= 1.8339
Yellow paramagnetic beads y = 1.11x-.06 .55 mg/mL .50 mg/mL OD420=.56, Units = 48890.86 .61 mg/mL OD420=.63, Units = 34878.04

Wednesday/Friday

Team Color purification method equation for BSA standard curve concentration of beta-gal stock concentration of purified beta-gal (wild type) activity of purified beta-gal (wild type) concentration of modified beta-gal (list modification below table) activity of modified beta-gal
Green Ni-agarose y = 0.6258x - 0.0197 3.39 mg/mL 0.473 mg/mL 0.0356 umol/min/mg 0.543 mg/mL (modified His) 0.0362 umol/min/mg
Purple
Red
Blue
Pink Ni-agarose y=.9833x-.0906 1.1899 mg/mL 0.2801 mg/mL 3.8357*10^8 umol/min/mg 0.3363 mg/mL (modified Glu) 5.2358*10^8 umol/min/mg
Yellow