Talk:BE.109:Protein engineering/Purifying beta-galactosidase

To compare the purification protocols you have tried today, each team in each lab section should enter their data into the following tables.

Tuesday/Thursday

Team Color	purification method	equation for BSA standard curve	concentration of beta-gal stock	concentration of purified beta-gal (wild type)	activity of purified beta-gal (wild type)	concentration of modified beta-gal (list modification below table)	activity of modified beta-gal
Green	Ni-agarose	y=.7133x - .0244	2.08 mg/ml	.29 mg/ml	27595 units	.158 mg/ml	1822 units
Purple	Paramagnetic Beads	OD-595 = 0.5556(Conc.) - 0.0012	4.19 mg/mL	0.0501 mg/mL	37.37 units	0.0506 mg/mL	34.87 units
Red	Ni-agarose	y=.7893x-0.0274	2.345 mg/mL	0.874 mg/mL	.076	.56 mg/mL	.085
Blue!	Ni-agarose	0.6709x-0.0002	3.75mg/mL	0.647	0.0318	0.322	0.233
Pink	Ni-agarose	y=1.2x+0.0002	2.332 mg/mL	0.111 mg/mL	OD 420= 0.7363 , Units = 2.6783	0.268 mg/mL	OD 420= 0.2173, Units= 1.8339
Yellow	paramagnetic beads	y = 1.11x-.06	.55 mg/mL	.50 mg/mL	OD420=.56, Units = 48890.86	.61 mg/mL	OD420=.63, Units = 34878.04

Wednesday/Friday

Team Color	purification method	equation for BSA standard curve	concentration of beta-gal stock	concentration of purified beta-gal (wild type)	activity of purified beta-gal (wild type)	concentration of modified beta-gal (list modification below table)	activity of modified beta-gal
Green	Ni-agarose	y = 0.6258x - 0.0197	3.39 mg/mL	0.473 mg/mL	0.0356 umol/min/mg	0.543 mg/mL (modified His)	0.0362 umol/min/mg
Purple
Red
Blue
Pink	Ni-agarose	y=.9833x-.0906	1.1899 mg/mL	0.2801 mg/mL	3.8357*10^8 umol/min/mg	0.3363 mg/mL (modified Glu)	5.2358*10^8 umol/min/mg
Yellow