Talk:20.109(S13):DNA extraction (Day2)

Each of you will isolate DNA from a bird stool sample. You will not prepare the same sample as your partner, so please read the table below carefully.

T/R Lab

Team color	Name	Sample #	Name	Sample #	AK or MA?
Red	Arturo	716	Nick	702	MA
Orange	Daphne	712	Stas	709	MA
Yellow	Dominique	739	Ebaa	664	AK
Green	Nikki	732	Jesse	405	AK
Blue	Justin	716	Noa	702	MA
Pink	Marisol	712	Tiffany	709	MA
Purple	Sarah	739	Mitali	664	AK
Grey/Platinum	Emily	732	Katie	405	AK

W/F Lab

Team color	Name	Sample #	Name	Sample #	AK or MA?
Red	Divya	716	Maria	702	MA
Orange	Katie	712	Amelia	709	MA
Yellow	Jenny W	739	Karinna	664	AK
Green	Kevin	732	Steve	405	AK
Blue	Emmanuel	716	Sachin	702	MA
Pink	Alice	712	Helen	709	MA
Purple	Zeinab	739	Jenny V	664	AK
Grey/Platinum	Eric	732	Shanthi	405	AK

Bird sample background

(1) Chain of storage

"Fecal collections are done using a sterile swab into a sterile tube of viral transport media, which contains a cocktail of antibiotics. The swab is swirled through the specimen, inserted into the vial, and stored on ice in a cooler while the remaining collections for that day are being done (usually takes about 2 hours all told back to the lab). Once at the lab, the samples are immediately transferred to -80C until processing. In the case of samples coming from Alaska (or any distant field site), the samples are moved directly from -80C to a dry shipper (maintains the temp at -150C) for air transport, then placed directly into a -80C when they arrive at our lab. Since all of these have been screened for avian influenza, the samples were thawed on ice and an aliquote was removed to assess the presence of AI. The sample is then refrozen at -80C."

(2) Locations

"These are all fecal samples, so they come from land near water. The Alaska samples are collected off of the fishing docks in the town of Cordova, AK on the Pacific. If you wanted to be more specific, I believe it's part of Prince William Sound. The MA samples are fecal pickups from Revere Beach."

(3) Expected species

"The Alaska gulls are primarily glaucous winged gulls, the Revere gulls are primarily herring gulls and ring billed gulls, with some greater black back gulls and the occasional bonaparte gull."

(4) Differences between MA and AK pick-ups?

"Other than different people at different locations at different times, no difference at all. The swabs, VTM, methods, etc are all the same."