qPCR data
T/R
The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (The latter is likely to be more reliable.)
Media:20109-S12_TR_qPCR.txt
W/F
The text file below contains all of the W/RF data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (The latter is likely to be more reliable.)
Media:20109-S12_WF_qPCR.txt
And here is a new set of data when point C3 (rises very quickly) is excluded when determining the "all" and "CN II" noisebands.
Media:20109-S12_WF_qPCR-corrected.txt
qPCR section-wide curves
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Raw amplification curves
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Log-scale amplification curves with noise threshold
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Log-scale 18S curves
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Log-scale CN II curves
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Log-scale CN I curves
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Melt curves for 18S samples
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Melt curves for CN II samples
Error creating thumbnail: Unable to save thumbnail to destination T/R section, Melt curves for CN II samples
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Raw amplification curves
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Log-scale amplification curves with noise threshold
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Log-scale 18S curves
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Log-scale CN II curves
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Log-scale CN I curves
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Melt curves for 18S samples
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Melt curves for CN II samples
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Melt curves for CN I samples
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Log-scale amplification curves with noise threshold, well C3 excluded
Error creating thumbnail: Unable to save thumbnail to destination W/F section, Log-scale CN II curves, well C3 excluded
Cross-group meetings
Scheme
Red 1/Pink 1
Red 2/Purple 1
Orange 1/Green 2
Orange 2/Blue 2
Yellow 2/Blue 1
Green 1/Purple 2
Pink 2/Yellow 1
T/R
Taleen/Ruisi
Jessica/Kristen
Nathan/Shambhu
Jeff/Fangdi from W/F section if possible
Angel/Michael
Esme/Cher
Maddy/Meena
W/F
Dorian/Lauren
Sarah/Dharshan
Sixing/Carlos
Samira/Nina
Melina/Isabella
Brian/Matt
Holly/Rachel
Fangdi/Jeff from T/R section if possible
(Brian and Carlos I often mix-up which one of you sits where, but please stick with the above for simplicity. Thanks!)