Talk:20.109(S12):Transcript-level analysis (Day6)
qPCR data
T/R
The text file below contains all of the T/R data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (The latter is likely to be more reliable.)
W/F
The text file below contains all of the W/RF data. Two different CP values are given. The first is calculated when all samples are thresholded at the same level. The second is calculated when a separate threshold is made for each primer set. (The latter is likely to be more reliable.)
And here is a new set of data when point C3 (rises very quickly) is excluded when determining the "all" and "CN II" noisebands.
Media:20109-S12_WF_qPCR-corrected.txt
qPCR section-wide curves
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/8/8e/S12_M3D5_TR_raw.jpg/400px-S12_M3D5_TR_raw.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/3/38/S12_M3D5_TR_noiseband.jpg/400px-S12_M3D5_TR_noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/7/7b/S12_M3D5_TR_18S-noiseband.jpg/300px-S12_M3D5_TR_18S-noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/5/5a/S12_M3D5_TR_CNII-noiseband.jpg/300px-S12_M3D5_TR_CNII-noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/4/40/S12_M3D5_TR_CNI-noiseband.jpg/300px-S12_M3D5_TR_CNI-noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/6/69/S12_M3D5_TR_18S-melt.jpg/300px-S12_M3D5_TR_18S-melt.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/7/79/S12_M3D5_TR_CNII-melt.jpg/300px-S12_M3D5_TR_CNII-melt.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/0/09/S12_M3D5_TR_CNI-melt.jpg/300px-S12_M3D5_TR_CNI-melt.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/7/7e/S12_M3D5_WF_raw.jpg/400px-S12_M3D5_WF_raw.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/0/02/S12_M3D5_WF_noiseband.jpg/400px-S12_M3D5_WF_noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/d/d2/S12_M3D5_WF_18S-noiseband.jpg/300px-S12_M3D5_WF_18S-noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/8/8f/S12_M3D5_WF_CNII-noiseband.jpg/300px-S12_M3D5_WF_CNII-noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/7/7a/S12_M3D5_WF_CNI-noiseband.jpg/300px-S12_M3D5_WF_CNI-noiseband.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/c8/S12_M3D5_WF_18S-melt.jpg/300px-S12_M3D5_WF_18S-melt.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/5/5c/S12_M3D5_WF_CNII-melt.jpg/300px-S12_M3D5_WF_CNII-melt.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/3/39/S12_M3D5_WF_CNI-melt.jpg/300px-S12_M3D5_WF_CNI-melt.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/3/34/S12_M3D5_WF_noiseband-corrected.jpg/400px-S12_M3D5_WF_noiseband-corrected.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/f/fb/S12_M3D5_WF_CNII-noiseband-corrected.jpg/300px-S12_M3D5_WF_CNII-noiseband-corrected.jpg)
Cross-group meetings
Scheme
Red 1/Pink 1
Red 2/Purple 1
Orange 1/Green 2
Orange 2/Blue 2
Yellow 2/Blue 1
Green 1/Purple 2
Pink 2/Yellow 1
T/R
Taleen/Ruisi
Jessica/Kristen
Nathan/Shambhu
Jeff/Fangdi from W/F section if possible
Angel/Michael
Esme/Cher
Maddy/Meena
W/F
Dorian/Lauren
Sarah/Dharshan
Sixing/Carlos
Samira/Nina
Melina/Isabella
Brian/Matt
Holly/Rachel
Fangdi/Jeff from T/R section if possible
(Brian and Carlos I often mix-up which one of you sits where, but please stick with the above for simplicity. Thanks!)