Talk:20.109(S12):Testing cell viability (Day3)

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T/R schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team color
2:05-2:30 1:05 Green
2:30-2:55 1:20 Yellow
2:55-3:20 1:45 Red
3:20-3:45 2:10 Blue
3:45-4:10 2:35 Pink
4:10-4:35 3:00 Purple
4:35-5:00 3:25 orange

W/F schedule

Staining protocol time is expected to be ~ 1 hour, leaving 25 min blocks of microscope time for each group.

Please come prepared to lab (i.e., having read the protocol), and feel free to arrive even earlier than the suggested times below.

Microscope time Arrival time (at latest!) Team Color
2:05-2:30 1:05 Purple
2:30-2:55 1:20 Pink
2:55-3:20 1:45 Blue
3:20-3:45 2:10 Green
3:45-4:10 2:35 Red
4:10-4:35 3:00 Orange
4:35-5:00 3:25 Yellow

T/R images

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M3D3 Green_TR_1, T/R
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M3D3 Green_TR_2, T/R
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M3D3 Green_TR_3, T/R
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TR_BLUE_CELL_1_CORE_UP, T/R
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TR_BLUE_CELL_2_CORE_DOWN, T/R
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TR_BLUE_CELL_3_CORE_DOWN, T/R
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TR_BLUE_CELL_4_CORE_UP, T/R
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TR_Yellow_1_noTGFB, T/R
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TR_Yellow_2_noTGFB, T/R
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TR_Yellow_3_noTGFB, T/R
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TR_Yellow_1_2TGFB, T/R
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TR_Yellow_2_2TGFB, T/R
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TR_Yellow_3_2TGFB, T/R
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TR_Orange_1_10 ug/mL TGFB Core, T/R
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TR_Orange_2_10 ug/mL TGFB Surface, T/R
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TR_Orange_3_10 ug/mL TGFB Surface, T/R
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TR_Orange_4_10 ug/mL TGFB Core, T/R
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Red FBS Core
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Red NBS
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TR_Pink_+HEPES_Surface
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TR_Pink_-HEPES_Surface


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TR_Purple_1_10TGFB, T/R
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TR_Purple_2_10TGFB, T/R
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TR_Purple_3_10TGFB, T/R
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TR_Purple_1_20TGFB, T/R
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TR_Purple_2_20TGFB, T/R
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TR_Purple_3_20TGFB, T/R
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WF_Red_TGFB_1, W/F
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WF_Red_TGFB_2, W/F
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WF_Red_TGFB_3, W/F
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WF_Red_BMP2_1, W/F
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WF_Red_BMP2_2, W/F
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WF_Red_BMP2_3, W/F
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WF_Yellow_Insulin_1, W/F
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WF_Yellow_Insulin_2, W/F
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WF_Yellow_Insulin_3, W/F
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WF_Yellow_noInsulin_1, W/F
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WF_Yellow_noInsulin_2, W/F
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WF_Yellow_noInsulin_3, W/F


W/F Images

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WF_Pink_2%FBS+IDT_First Image
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WF_Pink_2%FBS+IDT_Second Image
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WF_Pink_2%FBS+IDT_Third Image
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WF_Pink_10%FBS_First Image
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WF_Pink_10%FBS_Second Image
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WF_Pink_10%FBS_Third Image


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2.5%_Alginate_core_down, WF Purple
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1%_Alginate_core_down, WF Purple

[[Image:DSCN2129.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Core, WF Orange] [[Image:DSCN2134.JPG|thumb|left|400px|Stem Cells:Collagen TGFB Border, WF Orange] [[Image:DSCN2138.JPG|thumb|left|400px|Stem Cells:Collagen BMP Core, WF Orange]

No Transferrin, WF Green
Transferrin, WF Green
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With Insulin 1, WF Blue
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With Insulin 2, WF Blue
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With Insulin 3, WF Blue
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No Insulin 1 - core up, WF Blue
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No Insulin 2 - core down, WF Blue
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No Insulin 3, WF Blue