Talk:20.109(S12):Purify RNA and run affinity column (Day4)

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Sign-up for column protocols

T/R

You and your partner should propose a protocol in the table below.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.

Protocol# Group 8-12 % Wash # (partner 1) Wash # (partner 2) Notes (e.g., short justification for work, whether any other parameter was changed, etc.) Note changes to plan made on D4 (if any) here
0 Sample 5 5 34 Trying maximum wash number done in past. Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12.
1 Red 2% 4 24 following same protocol from Spring 2010 with the intention of comparing results.
2 Orange 1 4 24 To examine the enrichment at a lower concentration of 8-12.
3 Yellow 0.25 4 24 To get a sense for the level of enrichment at a low concentration and a high number of washes
4 Green 1 8 36 comparing a number of washes (8) that provides decent result with 36 to find maximum number of washes that provides a decent yield
5 Blue 10 10 30 30 is around max number done in the past. none yet
6 Pink 0.5 4 24 Trying a lower 8-12% (only 0.5) because it failed last year
7 Purple 10% 4 24 we want to repeat this experiment exactly as it was done in the past.


W/F

You and your partner should propose a protocol in the table below.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.

Protocol# Group 8-12 % Wash # (partner 1) Wash # (partner 2) Notes (e.g., short justification for work, whether any other parameter was changed, etc.) Note changes to plan made on D4 (if any) here
0 Sample 5 5 34 Trying maximum wash number done in past. Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12.
1 Red 10 14 20 Exploring the minimum wash number necessary to have sufficient enrichment. However, also interested in potentially doing 3 data points (if possible)!
2 Orange 0.5 4 24 Examine how very low concentration of '8-12' can effect enrichment.
3 Yellow 1 4 24 Examine enrichment using a low concentration of 8-12.
4 Green 50 4 24 Spring 2011 repeat
5 Blue 10 8 16 Spring 2010 repeat
6 Pink 10 2 34 Repeating from S11
7 Purple 2 20 20 Exploring effects of different temperatures (25C, 4C)