Talk:20.109(S12):Induce protein and evaluate DNA (Day5)

Induced pellet example

Examples of induced (left) and control (right) cell pellets

TR O/N Cultures and Gels

Below you should list the OD₆₀₀ values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group	OD WT	OD M124S/E67K/T79P	OD Chosen mutant	Pellets yellow?
Red
Orange	.067	.024	Mutant 1: .095 / Mutant 2: .065	Yes
Yellow	0.069	0.138	0.062	Yes
Green	0.062	0.150	0.140	Yes
Blue
Pink	0.071	0.317	0.119	Y: bright yellow green WT, F65D; dull/pale yellow T79P
Purple	0.0771	0.1352	0.0464	Yes

Gels

File:S12 M2D5 TR gel-Red.jpg

T/R Red

T/R Orange

T/R Yellow

T/R Green

T/R Blue NOTE: Gel Was Run Backwards

T/R Pink

File:S12 M2D5 TR gel-Purple.jpg

T/R Purple

WF O/N Cultures and Gels

Below you should list the OD₆₀₀ values of the induced cultures (at 1:10 dilutions), as well as your pellet observations. The teaching faculty will fill in this information for the cultures needing to be grown overnight.

Group	OD WT	OD M124S/E67K/T79P	OD Chosen mutant	Pellets yellow?
Red	.247	.047	.05	Yes
Orange	0.057	0.273	0.381	The pellets were yellow.
Yellow	0.387 (actually T79P)	0.118 (E67K)	0.095	Y
Green	0.396	0.105	0.094	Yes
Blue	0.366 (actually T79P)	0.109 (E67K)	0.102	Y
Pink	0.242 (actually T79P)	0.089 (E67K)	0.066	Yes
Purple	0.263 (actually T79P)	0.300 (M124S)	0.063	Yes

Gels

W/F Red

W/F Orange

W/F Yellow

W/F Green

W/F Blue -- NOTE: Lanes 8 and 9 are switched

W/F Pink

W/F Purple