Talk:20.109(S11):Wrap-up analysis and modelingb (Day8)
T/R
Please use the following order for all data: IPTG, IPTG/AHL, -/-, AHL.
Data should be written as: # units (% of AHL case). You can indicate the standard deviation of the data if it is known. (You may include one more significant figure than you believe (or not) for your classmates' reference, but don't go overboard!)
In notes you can explain if you are using week 1, week 4, or both data sets for the original system. Also specify which team's design you used, if your own did not produce colonies. Finally, definitely mention if some of your data points are untrustworthy due to saturation.
| Group | Miller units and % maximal (original) | Miller units and % maximal (modified) | Notes |
|---|---|---|---|
| Sample | 1320 (7.0%), 1470 (7.8%), 12,740 ± 20 (67%), 18,900 ± 300 | 700 (3.6%), 1280 (6.5%), 9100 ± 260 (46%), 19,600 ± 350 | week 4 data only |
| Blue | 1146.95 (3.4%), 1273.3 (3.8%), 31696.1 (95%), 33278.3 | 6111.7 (1176.6%), 6252.9 (1203.7%), 423.4 (81.5%), 519.5 | week 4 data only |
| Yellow | 2015(2.5%), 1843(2.3%), 61790(77%), 79800 | 27960(19%), 29930(20%), 138940(94%), 147470 | week 4 data only, -/- and AHL data may be unreliable due to saturation |
| Pink | 20008 (59%), 1061 (3.1%), 16659 (49.1%), 33903 | 7046 (3292%), 6083 (2842%), 265 (4.3%), 214 | week four data, modified data probably unreliable (used Green W/F design) |
| Purple | 7640, 7907, 116000, 108000 | 55239 (0.76%), 90264 (17.5%), 73772 (14.7%), 55060 (11.0%) | week four only |
| Red | 1573 (2.55%), 1551(2.52%), 48894 (79.5%), 61531 | 33756 (58.5%), 21459 (37.2%), 42282 (73.2%), 57740 | week four only |
| Green | 665 (15.8%), 1275(30.46%),10837(258%),4185 | 115(1.95%), 616(10.47%), 4610(78.36%), 5883 | week four only |
| Orange | 307.3(0.81%), 340.9(0.9%), 14101.1(37.36%), 37734.1 | 651.8(1.26%), 1689.7(3.27%), 39343.5(76.3%), 51531.3 | week 1 data only for original system. We ultimately used the T/R Blue group's design for the modified system because we did not get any colonies. |
W/F
Please use the following order for all data: IPTG, IPTG/AHL, -/-, AHL.
Data should be written as: # units (% of AHL case). You can indicate the standard deviation of the data if it is known. (You may include one more significant figure than you believe (or not) for your classmates' reference, but don't go overboard!)
In notes you can explain if you are using week 1, week 4, or both data sets for the original system. Also specify which team's design you used, if your own did not produce colonies. Finally, definitely mention if some of your data points are untrustworthy due to saturation.
| Group | Miller units and % maximal (original) | Miller units and % maximal (modified) | Notes |
|---|---|---|---|
| Sample | 1320 (7.0%), 1470 (7.8%), 12,740 ± 20 (67%), 18,900 ± 300 | 700 (3.6%), 1280 (6.5%), 9100 ± 260 (46%), 19,600 ± 350 | week 4 data only |
| Yellow | 1030 ± 20 (5.0%), 1000 ± 73 (4.9%), 17,100 ± 5200 (84%), 20,500 ± 73 | 156 ± 20 (0.92%), 172 ± 11 (1.0%), 7320 ± 690 (43%), 16,900 ± 90 | Previously posted values were a factor of 10 too high. (accidentally used 50 ul overnight culture rather than a 1:10 dilution) Week 4 data only |
| Purple | 294 (2.0%), 431 (2.9%), 12778 (86.2%), 14820 | 53 (0.87%), 49 (0.81%), 4088 (68.1%), 6001 | week four data only |
| Green | 3133 ± 1071 (4.17%), 2523 ± 683 (3.36%), 65516 ± 8381 (87.30%), 75045 ± 10079(100%) | 3437 ± 1202 (4.18%), 3621 ± 1236 (4.41%), 63504 ± 8436 (77.25%) 82206 ± 27188 (100%) | week 4 data only. Previously posted data were...just wrong. Upon realizing that the values calculated didn't make any sense even for the initial system, we did the calculations again and got more reasonable values. We're not really sure what happened the first time, but we think we averaged the wrong rows in Excel. |
| Blue | 2060 (3%), 2169 ± 99 (3%), 33495 ± 15253 (50%), 66165 ± 581(100%) | 2238 ± 30(3%), 14199 ± 6610(24%), 43769 ± 3169(76%), 57372 ± 741(100%) | week 4 data only. We redid the calculation because we forgot to subtract the blank sample from all other samples. |
| Pink | 1602 (2.7%), 1413 (2.4%), 42479 (73%), 57930 | 5789 (9.6%), 9837 (16.3%), 50430 (83.3%), 60517 | Week 1 and Week 4 data for the original construct |
| Red | 1656 (2.5%), 1695 (2.5%), 43077 (64%), 67583 | 357 ± 16 (2.1%), 432 ± 13 (2.5%), 11910 ± 334 (70%), 16990 ± 235 | Week 4 data only |
| Orange | 3240 (12.5%), 4380 (17%), 10680 (41%), 25900 (100%) | 390 (15%), 700 (27%), 1620 (62%), 2630(100%) | Week 4 data only; modified due to previous errors with Excel averaging. |
