Talk:20.109(S11):Purify RNA and run affinity column (Day4)

Sign-up for column protocols

You and your partner should propose a protocol in the table below.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.

Protocol#	Group	8-12 %	Wash # (partner 1)	Wash # (partner 2)	Note changes to plan here
0	Sample	5	5	15	Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12.
1	Red	.5	4	24	Starting with a lower 8-12 concentration.
2	Orange	50	8	16	Repeat high-percentage run because the decrease in enrichment with increasing number of washes intrigued us
3	Yellow	1	4	24	starting with a lower 8-12 concentration
4	Green	50	4	24	Repeating high-percentage run for statistical reliability.
5	Blue	2	4	32	Check to see at what limit does the trend of more washes contribute to the yield hold true.
6	Pink	2	4	24	Repeat from last year's experiment
7	Purple	10	8	32	Interested in seeing the effect of introducing greater number of washes

You and your partner should propose a protocol in the table below.

If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.

Protocol#	Group	8-12 %	Wash # (partner 1)	Wash # (partner 2)	Note changes to plan here
0	Sample	5	5	15	Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12.
1	Red	2	4	24	Repeat from last year's experiment
2	Orange	2	2	48	New experiment: Testing extremes of washing.
3	Yellow	50	8	16	Last year's experimenters observed a decrease in yield after more washes. Repeating to see if the break from the general trend is due to the conditions selected or experimenter variation.
4	Green	.25	4	24	New experiment, .25% pre-selection 8-12 to test lower limits of 8-12 recovery.
5	Blue	10	2	34	New experiment. Using 10% (we would have chosen 2%, but we suspect there could be contamination in our sample) but with extremes of washing.
6	Pink	10	8	16	Repeat from last year's
7	Purple	50	4	24	repeating last year's experiment that did not actually have 50%