Talk:20.109(S09):Start-up protein engineering (Day1)

TR lab

Team colour	Mutation X#Z	Primer (forward)	Silent mutation	New restriction site
Teaching sample	S101L	GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G	Ala 103	FspI
Red	D131G	G ATA AGG GAA GCA GAT ATC GGT GGT GAT GGC CAA GTT AAC TAT	Val 136	HpaI
Orange	E31I	C GCG CGC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG Ordered: GC ACC ATC ACC ACA AAG ATA CTG GGC ACC GTT ATG AGG	Leu 32	XcmI
Yellow	D133R	AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTA AAC TAT GAA GAG Ordered: AGG GAA GCA GAT ATC GAT GGT CGT GGC CAA GTT AAC TAT GAA GAG	Val 136	HpaI
Green	D133W	GG GAA GCA GAT ATC GAT GGT TGG GGC CAA GTT AAC TAT G	Val 414	HpaI
Blue	D58H	GAA GTC GAT GCG CAT GGC AAT GGA ACG ATT TAC	Ala 57	FspI
Pink	M144D	GAA GAG TTC GTA CAA GAC ATG ACA GCA AAG TAA GAA	Phe 419, Glu 418	EcoRI
Purple	D93P	CGA GAA GCT TTC CGT GTT TTT CCC AAG GAT GGG AAC GGC	Ala 88	HindIII
Grey	D22W	GCC TTC TCA TTA TTC GAC AAG TGG GGG GAC GGC AAC ATC ACC Ordered: GCC TTC TCA TTA TTC GAC AAG TGG GGA GAC GGC AAC ATC ACC	Gly 301	BsmBI

WF lab

Team colour	Mutation X#Z	Primer (forward)	Silent mutation	New restriction site
Teaching sample	S101L	GG AAC GGC TAC ATC CTC GCT GCG CAG TTA CGT CAC G	Ala 103	FspI
Red	D93H actual: D95H	GCA TTC CGT GTT TTT GAC AAG CAT GGG AAC GGA TAC ATC AGT GC	Gly 379 Ser 388	BaeI
Orange	E127K	GTT GAT GAA ATG ATC AGG AAA GCA GAT ATC GAT GGT GAT GGC	Ile 403	BclI
Yellow	L105S	C AGC GCT GCT CAG TCA CGT CAC GAC ATG ACA AAC Ordered: C AGC GCT GCT CAG TCA CGA CAC GTC ATG ACA AAC	Arg384	AflIII
Green	T79P	GA AAA ATG AAG GAC CCA GAC AGC GAA GAG G Ordered: see Green/Purple	Thr 79	PpuMI
Blue	E31R	ACC ATC ACC ACA AAG AGG CTG GGC ACC GTT ATG AGG	Leu 310	BseYI
Pink	M124S	GAA GTT GAT GAA TCG ATA AGG GAA GCA GAT ATC GAT GG	none	ClaI
Purple	T79P	GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC CGA GAA GC Ordered: see Green/Purple	PpuMI
Grey	L32D	GGC ACC ATC ACC ACA AAG GAA GAT GGT ACC GTT ATG AGG		KpnI
Green/Purple	T79P	GA AAA ATG AAG GAC CCA GAC AGC GAA GAG GAA ATC C	Thr 79	PpuMI