Talk:20.109(S08):DNA engineering/Restriction map and tissue culture (Day 6)
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Diagnostic Digests
TR lab
Many of the gels today did not have a visible ladder (the protocol was an old one that said to use only 5 μL - sorry about that). A few gels - for blue, and to a large extent purple and red - also seemed to indicate little DNA recovery from the minipreps.
| Team colour | Digest 1 | Digest 2 |
| Green | XhoI+EcoRV | BamHI+SalI |
| Purple | ||
| Red | EcoRV+NotI | BamHI+XhoI |
| Pink | SalI+EcoRV | NotI+EcoRV |
| Blue | NotI+EcoRV | XhoI+BamHI |
| Yellow | BamH1+Sal1 | EcoRV+Nsi1 |
WF lab
The gels were pretty bright today, including the ladder. For the blue group, the MW ladder is only faintly/partly visible on the original polaroid. The bands seem to fall b/w 4-5 Kbp for the most part, but it's hard to say for sure.
| Team colour | Digest 1 | Digest 2 |
| Green | XbaI/XhoI | BamHI/HsiI |
| Purple | ||
| Red | BamHI/XhoI | BamHI/EcoRV |
| Pink | BamHI/SalI | BamHI/NsiI |
| Blue | BamHI/XhoI | Nsi1/EcoRV |
| Yellow | XbaI/XhoI | BamHI/Nsil |
