Talk:20.109(S07): Measuring calcium in vitro

Data summary for T/R lab section

Team Color	equation for standard's best-fit line	R-squared	unknown solution tested	concentration (triplicate), units	average concentration
Red	Absorbance = 0.1254x - 0.0094	R^2 = 0.9995	Unknown Solution 3 (tap water)	[Ca2+] = 0.547, 0.560, 0.558 (mM)	Average = 0.555 mM
Blue	Absorbance = 0.0968x + 0.0591	R^2 = 0.8798	Trypsin	[Ca2+] = 0.282, 0.347, 0.309	Average = 0.313 mM
Purple	Absorbance = .0001[Ca2+] - .01 (unit: uM)	R^2 = .9998	Media	[Ca2+] = 3.890, 2.470, 2.550 (mM)	Average [Ca2+] = 2.970 mM
Yellow	Absorbance = 0.0846[Ca2+] + 0.0385	R^2 = 0.9877	unknown #3	0.0254 mM, 0.0254 mM, 0.0284 mM	Average 0.0264 mM
Green	Absorbance = 0.1002([CaCl2])	R^2 = 0.9644	Embryonic Stem Cell Medium	3.045, 1.906, and 2.076 (mM)	Average [Ca2+] = 2.342 mM
Pink	Absorbance = 0.1304[Ca2+] -0.0076	R^2 = 0.9934	unknown #2	[Ca2+] = 14.6204 mM, 13.2316 mM, 5.177 mM (disregarding last in average)	Average [Ca2+] = 13.926 mM

Data summary for W/F lab section

Team Color	equation for standard's best-fit line	R-squared	unknown solution tested	concentration (triplicate), units	average concentration
Red	y = 0.1419x - 0.0076	R2 = 0.9972	ESM	1.9098, 2.4489, 1.7900, mM	2.0496 mM
Blue	ABS = (8*10^-5)[Ca2+] + 0.1633, with [Ca2+] in microM	R^2 = .9665	trypsin	[negative/0]	[negative/0]
Purple	y = 0.0968x - 0.0128	R^2 = 0.9998	ESM	3.337, 3.032, 3.158 mM	3.176 mM
Yellow	Absorbance = [math]\displaystyle{ 0.01416[Ca^{2+}]-0.00715 }[/math], [Ca] in M	[math]\displaystyle{ R^2 = 0.9757 }[/math]	OptiMeM	0.8383, 1.2206, 0.3045 (mM)	.78778 mM
Green	ABS=0.1037*(Ca conc. in mM)	R^2=0.9891	media	2.266, 2.011, 2.170 mM	2.149 mM
Pink	Abs(612nm) = 0.1265(dilution in mM) - 0.0072	R2 = 0.9962	Media	2.333, 2.134, 2.108 mM	2.192 mM

Fluorescence Graphs

teacher data

W\F Blue Team

W\F Green Team

T/R Blue Team

T/R Purple Team

T/R Yellow Team

T/R Red Team

T/R Pink Team

T/Th Green Team

W/F Yellow

W/F Pink Team

W/F Purple Team

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