# Talk:20.109(S07): Measuring calcium in vitro

## Data summary for T/R lab section

Team Color equation for standard's best-fit line R-squared unknown solution tested concentration (triplicate), units average concentration
Red Absorbance = 0.1254x - 0.0094 R^2 = 0.9995 Unknown Solution 3 (tap water) [Ca2+] = 0.547, 0.560, 0.558 (mM) Average = 0.555 mM
Blue Absorbance = 0.0968x + 0.0591 R^2 = 0.8798 Trypsin [Ca2+] = 0.282, 0.347, 0.309 Average = 0.313 mM
Purple Absorbance = .0001[Ca2+] - .01 (unit: uM) R^2 = .9998 Media [Ca2+] = 3.890, 2.470, 2.550 (mM) Average [Ca2+] = 2.970 mM
Yellow Absorbance = 0.0846[Ca2+] + 0.0385 R^2 = 0.9877 unknown #3 0.0254 mM, 0.0254 mM, 0.0284 mM Average 0.0264 mM
Green Absorbance = 0.1002([CaCl2]) R^2 = 0.9644 Embryonic Stem Cell Medium 3.045, 1.906, and 2.076 (mM) Average [Ca2+] = 2.342 mM
Pink Absorbance = 0.1304[Ca2+] -0.0076 R^2 = 0.9934 unknown #2 [Ca2+] = 14.6204 mM, 13.2316 mM, 5.177 mM (disregarding last in average) Average [Ca2+] = 13.926 mM

## Data summary for W/F lab section

Team Color equation for standard's best-fit line R-squared unknown solution tested concentration (triplicate), units average concentration
Red y = 0.1419x - 0.0076 R2 = 0.9972 ESM 1.9098, 2.4489, 1.7900, mM 2.0496 mM
Blue ABS = (8*10^-5)[Ca2+] + 0.1633, with [Ca2+] in microM R^2 = .9665 trypsin [negative/0] [negative/0]
Purple y = 0.0968x - 0.0128 R^2 = 0.9998 ESM 3.337, 3.032, 3.158 mM 3.176 mM
Yellow Absorbance = $\displaystyle{ 0.01416[Ca^{2+}]-0.00715 }$, [Ca] in M $\displaystyle{ R^2 = 0.9757 }$ OptiMeM 0.8383, 1.2206, 0.3045 (mM) .78778 mM
Green ABS=0.1037*(Ca conc. in mM) R^2=0.9891 media 2.266, 2.011, 2.170 mM 2.149 mM
Pink Abs(612nm) = 0.1265(dilution in mM) - 0.0072 R2 = 0.9962 Media 2.333, 2.134, 2.108 mM 2.192 mM

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