Schedule
Sign up for a time to do flow cytometry in the table below. Please arrive at the time you choose and then expect to spend about 45 minutes in TC and another 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.
T/R
| Time block
|
Team 1
|
Team 2
|
Team 3
|
| I: 1-2:30 pm
|
Yellow Mustard
|
Red Cherries
|
Pink
|
| II: 2-3:30-pm
|
Blue team, best team
|
Green
|
Tiger Team!
|
| III: 3-4:30 pm
|
Purple team
|
|
|
W/F
| Time block
|
Team 1
|
Team 2
|
| I: 1-2:30 pm
|
Pinkle
|
Green
|
| II: 2-3:30-pm
|
Blue
|
|
Data Analysis
Reported data
To aid class-wide comparisons (and statistics!), please post the requested information in the table below in a timely fashion using three significant digits (ex. 3.72). For samples done in triplicate, please separate the three values with a semi-colon. Once you've completed your statistics, don't get over-excited with the number of significant figures you report! For example, "3.7" will suffice. Please describe the experimental test conditions in the form "0.2 ug D5 and 0.05 ug CUT D3." Be sure to include which enzyme was used to cut D3.
T/R
| Team color
|
% R2 Gated Mock
|
EGFP
|
Δ5
|
uncut Δ3
|
cut Δ3
|
Δ5+uncut Δ3
|
Δ5+cut Δ3
|
Exp A
|
Exp condition
|
Exp B
|
Exp condition
|
| Cherries
|
0.01
|
57.6
|
0.69
|
0.47
|
0.08
|
2.27; 2.69; 2.32
|
9.66; 10.14; 10.27 (PmeI)
|
7.63; 6.99; 7.89
|
0.1 ug D5 and 0.1 ug BamHI CUT D3
|
5.75; 7.45; 6.29
|
0.1 ug D5 and 0.5 ug BamHI CUT D3
|
| Tiger
|
0.00
|
46.7
|
0.06
|
0.01
|
0.00
|
2.00; 1.75; 1.33
|
11.7; 11.6; 11.6 (BamHI)
|
5.67; 5.17; 3.74
|
0.1 ug D5 and 0.1 ug Pme CUT D3
|
8.87; 11.4; 8.44
|
0.1 ug D5 and 0.4 ug BamHI CUT D3
|
| Mustard
|
0.00
|
54.35
|
0.00
|
2.21
|
1.72
|
6.76; 5.89; 5.00
|
4.99; 6.39; 7.54 (PmeI)
|
10.88; 9.25; 9.50
|
0.1 ug D5 and 0.25 ug PmeI CUT D3
|
4.10; 3.96; 3.77
|
0.1 ug D5 and 0.50 ug PmeI CUT D3
|
| Green
|
0.00
|
61.49
|
0.20
|
0.20
|
0.00
|
8.12;8.13;7.19
|
2.84;2.71;2.54 (PmeI)
|
4.68;4.53;4.07
|
0.1 ug D5 and 0.5 ug PmeI CUT D3
|
7.48;5.58;5.08
|
0.1 ug D5 and 0.05 ug PmeI CUT D3
|
| Blue, best
|
0.00
|
42.62
|
0.05
|
0.01
|
0.00
|
1.88; 2.43; 2.08
|
6.19; 8.73; 7.03 (PmeI)
|
14.06; 13.65; 16.94
|
0.1ug D5 and 0.1ug BamHI CUT D3
|
5.43; 5.48; 4.70
|
0.1ug D5 and 0.2ug PmeI CUT D3
|
| Pink
|
0.00
|
44.01
|
0.06
|
0.00
|
0.00
|
1.80; 1.64; 1.88
|
7.33; 6.66; 6.67 (BamH1)
|
5.69; 6.21; 5.71
|
0,1 D5 + 0,1 Pme1 cut D3
|
9.56; 11.65; 11.15
|
0,1 ug D5 + 0,5 BamH1 Cut D3
|
| Purple
|
0.00
|
54.35
|
blank
|
blank
|
0.00
|
blank; 2.21; 1.72
|
6.76; 5.89; 5.00 (BamH1)
|
4.99; 6.39; 7.54
|
0.1 ug D5 and 0.1 ug PmeI Cut D3
|
10.88; 9.25; 9.50
|
0.1 ug D5 and 0.5 ug BamH1 Cut D3
|
W/F
| Team color
|
% R2 Gated Mock
|
EGFP
|
Δ5
|
uncut Δ3
|
cut Δ3
|
Δ5+uncut Δ3
|
Δ5+cut Δ3
|
Exp A
|
Exp condition
|
Exp B
|
Exp condition
|
| Pinkle
|
3.19
|
41.34
|
0.67
|
0.04
|
0.01
|
2.53; 2.54; 2.41
|
8.58; 8.65; 8.47 (PmeI)
|
6.76; 5.93 (one missing)
|
0.1ug D5 and 0.05ug PmeI cut D3
|
3.20; 4.06; 4.25
|
0.1ug D5 and 0.025ug PmeI cut D3
|
| Blue
|
0.02
|
75.9
|
0.15
|
0.02
|
0.00
|
3.69; 3.56; 2.60
|
25.3; 24.6; 21.7 (BamHI)
|
15.2; 16.7; 11.2
|
0.1 ug D5 and 0.1 ug PmeI cut D3
|
16.3; 15.4; 15.6
|
0.1 ug D5 and 1.0 ug BamHI cut D3
|
| Green
|
0.00
|
57.05
|
0.05
|
0.03
|
0.02
|
2.65; 3.54; 3.04
|
7.03; 6.76; 6.93
|
20.69; 21.00; 23.06
|
0.1 ug of D5 vs. 0.5 ug of BamHI cut D3
|
15.68; 17.54; 14.60
|
0.1 ug of D5 vs. 1.0 ug of BamHI cut D3
|
