Talk:20.109(F12): Mod 1 Day 3 Agarose gel electrophoresis
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Here is an example of a gel with all the expected bands, as well as a gel with the needed bands excised.
You can use these in your assignments if you so choose.
![](https://s3-us-west-2.amazonaws.com/oww-files-public/1/1d/FragsMod1.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-public/2/22/Excisedfrag.jpg)
T/R lab
Red | Orange | Yellow | Green |
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Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
Row 1 legend:
- Red backbone
- Red fragment
- 1 kb ladder
- Orange backbone
- Orange fragment
- Yellow backbone
- Yellow fragment
- 1 kb ladder
- Green backbone
- Green fragment
Row 2 legend:
- Blue backbone
- Blue fragment
- 1 kb ladder
- Pink backbone
- Pink fragment
- 1 kb ladder
- Purple backbone
W/F Lab
Red | Orange | Yellow | Green |
Blue | Purple | Pink | |
And here's the gel with your recovered fragments/backbones, ready for ligation.
WF Legend:
- Green backbone
- Green fragment
- 1 kb ladder
- Blue backbone
- Blue fragment
- Pink backbone
- Pink fragment
- 1 kb ladder
- Purple backbone
- Purple fragment
You can upload the pre-purification gels for reference here
Please name your image files according to the convention below, and use the "upload file" link in the left-hand sidebar to upload them. If named correctly, the image file should automatically show up on this page!
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/c/c7/F12_M1D3_WF-Grn.jpg/250px-F12_M1D3_WF-Grn.jpg.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/6/69/F12_M1D3_WF-Blu.jpg/250px-F12_M1D3_WF-Blu.jpg)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/4/4b/F12_M1D3_WF-Prp.jpg/250px-F12_M1D3_WF-Prp.jpg.png)
![](https://s3-us-west-2.amazonaws.com/oww-files-thumb/8/8e/F12_M1D3_TR_PNK.jpg/250px-F12_M1D3_TR_PNK.jpg)