Talk:20.109(F11): Mod 1 Day 7 FACS analysis

Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.

team color	FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)	mock data	EGFP data	delta 5 data	delta 3 data	delta5+delta3	delta5+delta3(Pme)	delta5+delta3(Bam)	extra experiment (specify below)
Red	I = 12:30-2PM	0.00	50.51	0.00	0.00	1.91	4.98	3.89	not done
Orange	III = 2:30-4PM	0.00	35.48	0.00	0.01	1.11	1.98	1.86	not done
Yellow	I = 12:30-2PM	0.00	45.28	0.00	0.00	0.89	3.33	2.59	Not done
Green	I = 12:30-2PM	0.00	58.5	0.01	0.01	1.61	4.33	4.92	ethidium bromide (100 ng/ml), 0.24
Blue	II = 1:30-3PM	0.00	54.41	0.00	0.00	1.38	4.01	3.54	Not done
Pink	II = 1:30-3PM	0.00	46.04	0.01	0.00	1.19	3.97	2.69	Not done
Purple	II = 1:30-3PM	0.00	18.14	0.00	0.02	1.32	0.49	1.06	Not done

W/F FACS schedule

NOTE: data is %gated R2. If you'd like to know #s of cells assayed, please contact group directly.

team color	FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)	mock data	EGFP data	delta 5 data	delta 3 data	delta5+delta3	delta5+delta3(Pme)	delta5+delta3(Bam)	extra experiment (specify below)
Red	III = 2:30-4PM	0.00	64.35	0.01	0.02	0.01	0.02	0.02	not done
Orange	II = 1:30-3PM	0.00	wasn't saved :(	0.02	0.00	1.02	4.74	0.00	not done
Yellow	I = 12:30-2PM	0.00	56.33	0.00	0.00	2.14	6.34	6.50	0.89 (2:1 Δ5:Δ3, 0.15 μg DNA)
Green	II = 1:30-3PM	0.00	48.05	0.01	0.00	2.25	5.47	6.54	4.34 (50:50 of each Δ3 cut type added, 0.20 μg DNA total)
Blue	I = 12:30-2PM	0.00	17.99	0.00	0.00	1.36	8.55	6.00	1.40 (3:1 Δ5:Δ3, 0.20 μg DNA total)
Pink	III = 2:30-4PM	0.00	52.67	0.01	0.00	1.46	4.39	3.80	not done