Talk:20.109(F10): Mod 1 Day 6 Restriction map and tissue culture

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Part 1: Agarose Gel Electrophoresis

Your samples were run on a 1% agarose gel at 125 volts for 30-45 min. This is the order samples should have been loaded. If you did yours differently, please note under the image of your gel.

Lane Plasmid sample volume to load
1 pCX-NNX uncut 20 μl
2 cut with enzyme 1 20 μl
3 cut with enzyme 2 20 μl
4 cut with enzymes 1 and 2 20 μl
5 1 kb marker 10 μl
6 candidate 1 uncut 20 μl
7 cut with enzyme 1 20 μl
8 cut with enzyme 2 20 μl
9 cut with enzymes 1 and 2 20 μl
10 candidate 2 uncut 20 μl
11 cut with enzyme 1 20 μl
12 cut with enzyme 2 20 μl
13 cut with enzymes 1 and 2 20 μl
14 1 kb marker 10 μl

T/R lab

Below is an image of your gel of the diagnostic digest. Please fill in the enzymes you used and what bands you expected for your double digest

Red Orange Yellow Green
Enzymes:

Expected double digest bands:

Enzymes:

Expected double digest bands:

Enzymes:

Expected double digest bands:

Enzymes:

Expected double digest bands:

Blue Pink Purple Gray
Enzymes:

Expected double digest bands:

Enzymes:

Expected double digest bands:

Enzymes:

Expected double digest bands: