Sign up for time on the FACS. Please arrive at the time you choose and then expect to spend 45 minutes in TC and 45 minutes at FACS. TWO or THREE teams should sign up for each time slot.

T/R FACS schedule

team color	FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)
Red	II
Orange
Yellow
Green	II
Blue	I
Pink	I
Purple	I
Silver

T/R FACS Data

Frequency of Parent for Green Cells

Sample	Red	Orange	Green	Blue	Pink	Purple	Silver
Mock	0	xx	xx	xx	xx	xx	xx
EGFP	5.82	xx	xx	xx	xx	xx	xx
D5	0	xx	xx	xx	xx	xx	xx
D3	0	xx	xx	xx	xx	xx	xx
D5:D3 1:1	0.25	xx	xx	xx	xx	xx	xx
D5:D3 1:0.5	0.061	xx	xx	xx	xx	xx	xx
D5:D3 1:2	0.13	xx	xx	xx	xx	xx	xx
D5:D3 1:10	0.017	xx	xx	xx	xx	xx	xx

T/R lab

Below are links to the flow data from today.

Red	Orange	Yellow	Green

Blue	Purple	Pink

I pulled all the numbers from each image and put them on one jpg. You'll have to zoom in and scroll to see the numbers that you want. All of the graphs are combined into one image here if it is easier for you to compare data that way.

Don't forget to average a few groups together. Yellow and Orange, you were suppose to average yours together, but neither of you got 'ideal' data, so each of you average yours with two others that had controls like we expected.

In the first 3 groups from today, remember that we only did Pink's D3 and D5 control, and we assumed that Blue and Purple worked (not a good idea normally, but we were crunched for time). Everyone think about (and write about) why it was important to include the D3 and the D5 controls.

The percentage of parent for the green cells is the percent of live cells that is green. This is the important number!

W/F FACS schedule

team color	FACS time block (I = 12:30-2PM, II = 1:30-3PM, III = 2:30-4PM, as needed IV = 3:30-5PM)
Red	I
Orange
Yellow
Green	III
Blue	Time block II
Pink	II
Purple	II
Silver

W/F Lab

Don't forget to average a few groups together. Everyone think about (and write about) why it was important to include the D3 and the D5 controls. The percentage of parent for the green cells is the percent of live cells that is green. This is the important number!