Talk:20.109(F09): Mod 1 Day 6 Restriction map and tissue culture
Part 1: Agarose Gel Electrophoresis
Your samples were run on a 1% agarose gel at 125 volts for 30-45 min. This is the order samples should have been loaded. If you did yours differently, please note under the image of your gel.
| Lane | Plasmid | sample | volume to load |
|---|---|---|---|
| 1 | pCX-NNX | uncut | 20 μL |
| 2 | cut with enzyme 1 | 20 μL | |
| 3 | cut with enzyme 2 | 20 μL | |
| 4 | cut with enzymes 1 and 2 | 20 μL | |
| 5 | 1 kb marker | 10 μL | |
| 6 | candidate 1 | uncut | 20 μL |
| 7 | cut with enzyme 1 | 20 μL | |
| 8 | cut with enzyme 2 | 20 μL | |
| 9 | cut with enzymes 1 and 2 | 20 μL | |
| 10 | candidate 2 | uncut | 20 μL |
| 11 | cut with enzyme 1 | 20 μL | |
| 12 | cut with enzyme 2 | 20 μL | |
| 13 | cut with enzymes 1 and 2 | 20 μL | |
| 14 | 1 kb marker | 10 μL |
T/R lab
Below is an image of your gel of the diagnostic digest. Please fill in the enzymes you used and what bands you expected for your double digest
| Red | Orange | Yellow | Green |
| Enzymes: BamHI & XhoI
Expected double digest bands: 1180 and 4158 |
Enzymes: BamHI & XhoI
Expected double digest bands: 1180 and 4160 |
Enzymes: BamHI, SalI
Expected double digest bands: 3800, 1600 |
Enzymes: XhoI, EcoRV
Expected double digest bands: 547bp, ~4797bp |
| Blue | Purple | Pink | |
| Enzymes: BamHI & Xho1
Expected double digest bands: 1172bp & 4170 bp |
Enzymes: BamHI & XhoI
Expected double digest bands: 1184bp & 4160bp |
Enzymes: BamHI & Xho1
Expected double digest bands: 1198bp & 4160bp |
|
| Error creating thumbnail: File missing |
W/F lab
Below is an image of your gel of the diagnostic digest. Please fill in what enzymes you used and the bands you expected for your double digest
| Red | Green | Blue | Purple |
| Enzymes: Xho1 and BamH1
Expected double digest bands: 4160 and 1220 |
Enzymes: BamH1 and xho1
Expected double digest bands: 1629 and 3701 |
Enzymes: BamHI and NotI
Expected double digest bands: 1214 and 4132 |
Enzymes: BamHI and Xho1
Expected double digest bands: 1180 and 4160 |
| Error creating thumbnail: File missing | |||
| Pink | |||
| Enzymes: BamH1 XhoI
Expected double digest bands: 1629 and 3701 |
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